Phlebotomy Plan

Order of draw trainer

Short answer

The CLSI order of draw is blood culture tube first, then the sodium citrate tube with a light blue closure, then serum tubes with clot activator and gel in red, red-speckled or gold, then heparin in dark, light or speckled green, then EDTA in lavender, pearl or pink, and the sodium fluoride and potassium oxalate glycolytic inhibitor tube in gray last. The sequence prevents additive carryover from altering the next test.

The complete order of draw for evacuated tube venipuncture, per CLSI GP41.

Pool for this variant: 9 items. A real draw is 6.

Sequence per CLSI's published statement of GP41. Tube closure colours vary between manufacturers — WHO instructs verifying them with your local laboratory. Inversion counts are deliberately not shown: neither CLSI's article nor WHO states a per-tube count, and NHA task 3N points at the tube manufacturer's guidelines.

How this works

The sequence comes from the Clinical and Laboratory Standards Institute's own published statement of the order in GP41, not from a manufacturer wall chart. CLSI is the body the NHA CPT test plan names at knowledge statement k25 and again at task 2O. GP41 itself is not free to read, so nothing beyond CLSI's published article is restated here. Additive names, their mode of action and the tests each tube serves are taken from the WHO phlebotomy guidelines; note that WHO's own Table 2.3 sequence is based on the 2003 NCCLS consensus and is superseded, so this trainer follows CLSI for the order and WHO only for the additive detail. The capillary variant follows WHO section 7.1.3, which places the haematology specimen first, then chemistry, then blood bank. Colour codes vary between manufacturers, which is why each tube is labelled by its additive as well as its closure.

Worked examples

Drawing a coagulation panel, a CBC and a glucose means light blue, then lavender, then gray. Drawing EDTA before citrate is the classic error: both additives work by binding calcium, so EDTA carried into the citrate tube adds an uncontrolled anticoagulant to a test that depends on a precise citrate-to-blood ratio. CLSI's exception is the other half of the rule — only blood culture tubes, glass nonadditive serum tubes, or plastic serum tubes without a clot activator may be collected before the coagulation tube.

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Primary references

  1. Order of Blood Draw Tubes and Additives — Clinical and Laboratory Standards Institute (CLSI) · primary · retrieved 2026-09-22, verified 2026-09-22
  2. NHA Certified Phlebotomy Technician (CPT) — Test Plan for the CPT Exam — National Healthcareer Association (NHA) · primary · retrieved 2026-09-22, verified 2026-09-22
  3. WHO Guidelines on Drawing Blood: Best Practices in Phlebotomy — World Health Organization · government · retrieved 2026-09-22, verified 2026-09-22