Phlebotomy Plan

CPT practice question: Routine Blood Collections

Routine Blood CollectionsDifficulty 4/5

CLSI states that only certain tubes may be collected before the coagulation tube. Which describes them?

  1. AAny tube at all, provided the coagulation tube is filled completely to its stated volume
  2. BBlood culture tubes, glass nonadditive serum tubes, or plastic serum tubes without clot activator
  3. CBlood culture tubes, heparin tubes with gel, or plastic serum tubes containing a clot activator
  4. DEDTA tubes with gel separator, glass nonadditive serum tubes, or any gray closure tube
Show answer and rationale

Correct: B. This is the exception CLSI spells out explicitly, and it is exactly what a colour-list recitation misses. A clot activator is the disqualifier: a plastic serum tube may precede the coagulation tube only if it has none.

  • C — A clot activator is precisely what disqualifies a serum tube from preceding the coagulation tube.
  • D — EDTA binds calcium and would corrupt a coagulation result; it never precedes the citrate tube.
  • A — Full filling matters for the citrate ratio but does not override the carryover rule.

Concept being tested

28 of the 100 scored items and the second largest domain. Equipment selection and verification, needle gauges and lengths, adapting technique for patients with burns, dementia or bleeding disorders, tourniquet application and removal, site selection by observation and palpation, antisepsis, anchoring the vein, needle insertion angle, following the order of draw for both venipuncture and capillary collection, recognising and responding to complications such as syncope, hematoma, petechiae and nerve pain, needle safety devices, tube inversion, dermal puncture, post-procedural care and labelling. — 28% of the exam. Study guide for Routine Blood Collections.

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Primary references

  1. Order of Blood Draw Tubes and Additives — Clinical and Laboratory Standards Institute (CLSI) · primary · retrieved 2026-09-22, verified 2026-09-22
Last reviewed: 2026-10-07Reviewed by: Maria Torres, CPT, RMA (AMT), Clinical laboratory educator & venipuncture safety reviewHow we verify facts