Phlebotomy Plan

CPT practice question: Routine Blood Collections

Routine Blood CollectionsDifficulty 3/5

What is the reason the order of draw exists?

  1. ALaboratory instruments reject specimens whose tubes arrive in the wrong sequence
  2. BDrawing out of sequence increases the volume of blood required from the patient
  3. CAdditive carried from one tube into the next can alter the result of the later test
  4. DTubes filled out of sequence will not develop sufficient vacuum to draw fully
Show answer and rationale

Correct: C. The sequence prevents cross-contamination of additives between tubes. It runs from the tube most vulnerable to contamination toward the additives most likely to interfere.

  • D — Vacuum in an evacuated tube is set at manufacture and does not depend on draw order.
  • A — Instruments analyse what they are given; they cannot detect the order of collection.
  • B — Sequence does not change the volume required for each individual test.

Concept being tested

28 of the 100 scored items and the second largest domain. Equipment selection and verification, needle gauges and lengths, adapting technique for patients with burns, dementia or bleeding disorders, tourniquet application and removal, site selection by observation and palpation, antisepsis, anchoring the vein, needle insertion angle, following the order of draw for both venipuncture and capillary collection, recognising and responding to complications such as syncope, hematoma, petechiae and nerve pain, needle safety devices, tube inversion, dermal puncture, post-procedural care and labelling. — 28% of the exam. Study guide for Routine Blood Collections.

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Primary references

  1. Order of Blood Draw Tubes and Additives — Clinical and Laboratory Standards Institute (CLSI) · primary · retrieved 2026-09-22, verified 2026-09-22
  2. WHO Guidelines on Drawing Blood: Best Practices in Phlebotomy — World Health Organization · government · retrieved 2026-09-22, verified 2026-09-22
Last reviewed: 2026-10-07Reviewed by: Maria Torres, CPT, RMA (AMT), Clinical laboratory educator & venipuncture safety reviewHow we verify facts